COSMID: CRISPR Search with Mismatches, Insertions and/or Deletions

Target Genome

Query Sequence

Enter Guide Strand Sequence in text window below (min 10 max 55 nt)

Search Options

Add PAM suffix: suffix can be NGG, NAG, NRG, or left empty (no appended)

Allowed indels and mismatch:

	0	1	2	3	(number of allowed mismatches)
No indels
1-base Del
1-base Ins

PCR Primer Design Options

Perform Primer Design According to the Following Setting.

Primer design parameter templates:

Parameter setting:

Min Separation Uncleaved to Cleaved:

Min Cleavage Product Size Difference:

Min Amplicon Length:

Max Amplicon Length:

Optimal Amplicon Length:

If you use COSMID, please cite: COSMID: A Web-based Tool for Identifying and Validating CRISPR/Cas Off-target Sites. TJ Cradick, P Qiu, CM Lee, EJ Fine, G Bao. Molecular Therapy—Nucleic Acids 3 (12), e214. Link
The ability of CRISPR to cut sites with indels described here: Nucleic Acids Research, Link
COSMID is made available to non-profit academic/research institutions at free-of-cost for the purpose of internal research and development only. For-profit institutions or companies can obtain access to COSMID via a license agreement. For further details, please contact Peng Qiu at peng.qiu@bme.gatech.edu